ATCC medium: 1194 DV medium

	Compound	Quantity
1	NaCl	1.8 g
2	KCl	60.0 mg
3	MgSO4 x 7H2O	0.5 g
4	NaNO3	50.0 mg
5	CaCl2	10.0 mg
6	TRIS	100.0 mg
7	Na2SiO3 x 9H2O	20.0 mg
8	K2HPO4	3.0 mg
9	Metal Mix (see below)	3.0 mL
10	FeCl2	0.01 mg
11	NTA	10.0 mg
12	Vitamin B12	0.3 mcg
13	Vitamin Mix (see below)	0.1 mL
14	Charcoal-filtered seawater	100.0 mL

Autoclave at 121C for 15 minutes without the Vitamin Mix. Aseptically add Vitamin Mix when cool and dispense in 5.0 ml quantities to 20 x 150mm screw-capped test tubes.

Metal Mix:

	Compound	Quantity
1	EDTA	25.0 mg
2	FeCl2	0.25 mg
3	H3BO3	0.5 mg
4	MnCl2	1.0 mg
5	ZnCl2	125.0 mcg
6	CoCl2	25.0 mcg
7	Distilled water	25.0 mL

Vitamin Mix:

	Compound	Quantity
1	Thiamine x HCl	20.0 mg
2	Biotin	50.0 mcg
3	B12	5.0 mcg
4	Folic acid	0.025 mg
6	p-Aminobenzoic acid	1.0 mg
7	Nicotinic acid	10.0 mg
8	thymine	50.0 m
9	Inositol	100.0 mg
10	Putrescine x 2HCl	4.0 mg
11	Riboflavin	0.5 mg
12	Pyridoxamine x 2HCl	2.0 mg
13	Orotic acid	26.0 mg
14	Flinic acid	20.0 mcg
15	Calcium D-(+)-pantothenate	10.0 mg
16	Pyridoxine x HCl	4.0 mg
17	Distilled water	100.0 mL