DunaBase: Dunaliella Database

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ATCC medium: 1194 DV medium


Compound Quantity
1 NaCl 1.8 g
2 KCl 60.0 mg
3 MgSO4 x 7H2O 0.5 g
4 NaNO3 50.0 mg
5 CaCl2 10.0 mg
6 TRIS 100.0 mg
7 Na2SiO3 x 9H2O 20.0 mg
8 K2HPO4 3.0 mg
9 Metal Mix (see below) 3.0 mL
10 FeCl2 0.01 mg
11 NTA 10.0 mg
12 Vitamin B12 0.3 mcg
13 Vitamin Mix (see below) 0.1 mL
14 Charcoal-filtered seawater 100.0 mL


Autoclave at 121C for 15 minutes without the Vitamin Mix. Aseptically add Vitamin Mix when cool and dispense in 5.0 ml quantities to 20 x 150mm screw-capped test tubes.

Metal Mix:


Compound Quantity
1 EDTA 25.0 mg
2 FeCl2 0.25 mg
3 H3BO3 0.5 mg
4 MnCl2 1.0 mg
5 ZnCl2 125.0 mcg
6 CoCl2 25.0 mcg
7 Distilled water 25.0 mL


Vitamin Mix:


Compound Quantity
1 Thiamine x HCl 20.0 mg
2 Biotin 50.0 mcg
3 B12 5.0 mcg
4 Folic acid 0.025 mg
6 p-Aminobenzoic acid 1.0 mg
7 Nicotinic acid 10.0 mg
8 thymine 50.0 m
9 Inositol 100.0 mg
10 Putrescine x 2HCl 4.0 mg
11 Riboflavin 0.5 mg
12 Pyridoxamine x 2HCl 2.0 mg
13 Orotic acid 26.0 mg
14 Flinic acid 20.0 mcg
15 Calcium D-(+)-pantothenate 10.0 mg
16 Pyridoxine x HCl 4.0 mg
17 Distilled water 100.0 mL

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