Dunaliella medium from CCALA

Mix 20 ml of the stock macroelements solution with 910 ml of artificial seawater and 30 ml of seawater soil extract.

	Macroelements	100 ml
1	KNO3	1.0 g
2	K2HPO4	0.1 g
3	MgSO4 x 7H2O	0.1 g

	Artificial seawater	1000 ml
1	NaCl	60 g
2	MgSO4 x 7H2O	10 g
3	KCl	1.5 g
4	CaSO4	2.0 g

Seawater soil extract: 1/3 of 6 litre flask fill with garden or leaf soil (not too great humus or clay content), add artificial seawater until it stands 5cm above the soil. Sterilize by heating in a steamer one hour, twice in a 24h interval. Separate the decanted extract by centrifugation. Fill into small containers, autoclave 20 minutes/1 atmosphere; store in refrigerator.

Reference: Schlösser (1982)