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CCAP: JM (Jaworski's Medium)
Freshwater algae
| Stocks | Per 200 mL | |
|---|---|---|
| 1 | Ca(NO3)2 x 4H2O | 4.0 g |
| 2 | KH2PO4 | 2.48 g |
| 3 | MgSO4 x 7H2O | 10.0 g |
| 4 | NaHCO3 | 3.18 g |
| 5 |
EDTAFeN |
0.45g |
| EDTANa2 | 0.45 g | |
| 6 |
H3BO3 |
0.496 g |
|
MnCl2 x 4H20 |
0.278 g | |
| (NH4)6Mo7O24 x 4H2O | 0.20 g | |
| 7 |
Cyanocobalamin |
0.008g |
| ThiamineHCl | 0.008g | |
| Biotin | 0.008 g | |
| 8 | NaNO3 | 16.0g |
| 9 | Na2HPO4.12H2O | 7.2 g |
Medium per Liter
Stock Solution 1-9, 1 mL each
Make up to 1 litre with deionized water. For agar, add 15.0 g per litre of *Bacteriological Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.
*Supply: Unipath Ltd, Wade Road, Basingstoke, Hants RG24 0PW, UK