CCAP: SE1 (Soil Extract 1)

Used in media for marine algae

Soil is prepared as above. Air-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and filtered through Whatman No 1 filter paper, then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (eg 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.