CCAP: SE2 (Soil Extract 2)

Used in media for freshwater and terrestrial protozoa

Soil is prepared as above. 105 g of air-dried sieved soil and 660 ml of deionized water are placed in a 1 litre bottle and autoclaved once at 15 psi for 15 minutes, then again after 24 hours. The contents of the bottle are left to settle (usually for at least a week) and then the supernatant is decanted and filtered. The final pH should be 7.0 - 8.0.