CCMP: Blue Star agar

Prepare Prov medium and include Na₂SiO₃ · 9H₂O (final medium concentration of 1.07 x 10^-4 M; use 1 mL of a 30 g/L dH₂O stock solution). Dissolve 9g Bacto-agar (heat and mix). For test tubes, dispense dissolved agar medium into tubes, autoclave, and then cool with tubes slanted at an angle. For Petri plates, autoclave in a flask, cool almost to the gelling point, and then aseptically dispense into sterile Petri plates.

Note: Agar concentration can be varied to produce softer or firmer substrates.