CCMP: f/2 Medium

(Guillard & Ryther 1962, Guillard 1975)

To 950 mL filtered seawater add:

Compound	Quantity	Stock solution	Molar Concentration in final Medium
NaNO₃	1mL	75 g/L dH₂O	8.83 x 10^-4 M
NaH₂PO₄ x H₂O	1mL	5 g/L dH₂O	3.63 x 10^-5 M
Na₂SiO₃ x 9H₂O*	1ML *	30 g/L dH₂O*	1.07 x 10^-4 M*
f/2 trace metal solution	1 mL	(see recipe below)	-
f/2 vitamin solution	0.5 mL	(see recipe below)	-

Make final volume up to 1 L with filtered seawater and autoclave.

*Note: Autoclaved f/2 medium produces extensive silica precipitate. We delete silicate when it is not required by the alga (see f/2-Si medium below).