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CCMP: f/2 Trace Metal Solution from CCMP

(Guillard & Ryther 1962, Guillard 1975)

To 950 mL dH2O add:

Compound Quantity Stock Solution Molar concentration
in final Medium
FeCl3 x 6H2O 3.15 g - 1 x 10-5 M
Na2EDTA x 2H2O 4.36 g - 1 x 10-5 M
CuSO4 x 5H2O   1 mL 9.8 g/L dH2O 4 x 10-8 M
Na2MoO4 x 2H2 1 mL 6.3 g/L dH2O 3 x 10-8 M
ZnSO4 x 7H2O 1 mL 22.0 g/L dH2O 8 x 10-8 M
CoCl2 x 6H2O 1 mL 10.0 g/L dH2O 5 x 10-8 M
MnCl2 x 4H2O 1 mL 180.0 g/L dH2O 9 x 10-7 M


Make final volume up to 1L with dH2O. Autoclave.

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