CCMP: f/2 Vitamin Solution

(Guillard & Ryther 1962, Guillard 1975)

To 950 mL dH₂O add:

Compound	Quantity	Stock Solution	Molar concentration in final solution
Vitamin B12 (cyanocobalamin)	1 mL	1.0 g/L dH₂O	1 x 10^-10 M
Biotin	10 mL	0.1 g/L dH₂O	2 x 10^-9 M
Thiamine x HCl	200 mg		3 x 10^-7 M

Make final volume up to 1 L with dH₂O. Autoclave and store in refrigerator.

Note: Vitamin B12 and biotin are obtained in a crystalline form. When preparing the vitamin B12 stock solution, allow for approximately 11% water of crystallization (for each 1 mg of Vitamin B12, add 0.89 mL dH₂O). When preparing the biotin stock solution, allow for approximately 4% water of crystallization (for each 1 mg of biotin, add 9.6 mL dH₂O).