SAG: Basal Medium (= ES "Erddekokt + Salze")

Source: SAG

	Compound	Stock Solution [g/100 ml]	Nutrient Solution [ml]
1	KNO3	1	20
2	K2HPO4	0.1	20
3	MgSO4 x 7H2O	0.1	20
4	Soil extract *	-	30
5	Micronutrient solution **	-	5
6	Distilled Water	-	905

* Preparation of soil extract: Fill a 6 liter flask one third with garden or leaf soil of medium, but not too great humus content which does not contain fertilizers or plant protective agents. Success of soil extract depends on selection of suitable soils. Those with high clay content are usually less satisfactory. Add deionized water until it stands 5 cm above the soil and sterilize by heating in a steamer for one hour twice in a 24 h interval. Separate the decanted extract from particles by centrifugation. Fill into small containers of stock solution each of a size appropriate to making a batch of media, autoclave for 20 min at 121 °C and store in the refrigerator.

** Preparation of the micronutrient solution:

	Compound	Stock Solution [g/100ml]	Applied Solution [ml]
1	ZnSO4 x 7H2O	0.1	1
2	MnSO4 x 4H2O	0.1	2
3	H3BO3	0.2	5
4	Co(NO3)2 x 6H2O	0.02	5
5	Na2 MoO4 x 2H2O	0.02	5
6	CuSO4 x 5H2O	0.0005	1
7	Distilled water	-	981
8	FeSO4 x 7H2O	-	0.7g
9	EDTA (Titriplex III, Merck)	-	0.8g

Autoclave the components separately in two solutions which are united after cooling.

Solution I: 881 ml distilled water + stock solutions of salts without FeSO₄ + 0.4 g EDTA

Solution II: 100 ml distilled water + 0.7 g FeSO₄ + 0.4 g EDTA