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SAG: Dunaliella acidophila Medium (= Dun. Ac.)
(from M. Puncocharova, Cult. Coll. Algae, Dept. Bot. Univ. Prague, CZ)
| Compound |
Amount added |
|
|---|---|---|
| 1 | MgSO4 x 7H2O | 25.00 g |
| 2 | NH4NO3 | 0.25 g |
| 3 | K2HPO4 | 0.075 g |
| 4 | CaCl2 x 2H2O | 0.012 g |
| 5 | peat extract (see media 7 and 1) | 5.0 ml |
| 6 | micronutrient solution (see medium 1) | 5.0 ml |
| 7 | distilled water | 990.0 ml |
Adjust to pH 1.5-1.8 with 1N H2SO4.
Add vitamin B12 (5 x 10-6g/l) in sterile solution after autoclaving.