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SAG: Seawater Medium (= SWES "Seewasser + Erddekokt + Salze")
| Compound |
Stock Solution [g/100ml] |
Nutrient Solution [ml] |
|
|---|---|---|---|
| 1 | KNO3 | 1 | 20 |
| 2 | K2HPO4 | 0.1 | 20 |
| 3 | MgSO4 x 7H2O | 0.1 | 20 |
| 4 | Soil Extract (see medium 1) | - | 30 |
| 5 | Micronutrient solution (see medium 1) | - | 5 |
| 6 | Filtered seawater | - | 905 |
Sterilize for 1 h in a steamer or autoclave if axenic culture is desired. Autoclave soil extract separately and unite after cooling. Add vitamin B12 (5 x 10-6g) in sterile solution.