UTEX: Erdschreiber Medium

(Mary Parke, pers. comm.)

Modified from the original Plymouth seawater recipe. General purpose marine medium for xenic cultures [for bacteria-free cultures see reprints in Rosowski & Parker (1971)].

Preparation: Prepare separate tubes to contain 0.2 g NaNO₃/10 mL H₂O and 0.03 g Na₂HPO₄ . 7H₂O/10 mL H₂O. Autoclave and store at 10° C.

Erdschreiber solution: to 1000 mL of pasteurized, filtered seawater (according to UTEX), add the following:

	Compound	Quantity
1	NaNO₃ stock solution	1 tube
2	Na₂HPO₄ . 7H₂O stock solution	1 tube
3	Vitamin B12 (15 x 10^-6 g/100 ml H₂O)	1 ml
4	PIV metal solution	12 ml
5	Soilwater (GR+) supernatant (according to UTEX)	50 ml