UTEX: PIV metal solution
To 1000 ml of glass-distilled water, add 0.750 g of Na2EDTA, and dissolve fully. Add the following salts in the amounts indicated:
| Compound | Quantity | |
|---|---|---|
| 1 | FeCl3 x 6H2O | 97 mg |
| 2 | MnCl2 x 4H2O | 41 mg |
| 3 | ZnCl2 | 5 mg |
| 4 | CoCl2 x 6H2O | 2 mg |
| 5 | Na2MoO4 x 2H2O | 4 mg |
For marine algae that require salinities less than 30 ppt, the basic erdschreiber medium is combined with glass-distilled water prior to inoculating the new culture, such that 1/2-erdschreiber combines erdschreiber medium and distilled water 1:1; 2/3-erdschreiber combines erdschreiber medium and distilled water 2:1, etc.
Reference: UTEX
